Nucleotide excision repair in Escherichia coli
نویسندگان
چکیده
منابع مشابه
The Role of Nucleotide Excision Repair in Restoring Replication Following UV-Induced Damage in Escherichia coli
Following low levels of UV exposure, Escherichia coli cells deficient in nucleotide excision repair (uvrA6 mutants) recover and synthesize DNA at near wild type levels, an observation that formed the basis of the post-replication recombination repair model. In this study, we characterized the DNA synthesis that occurs following UV-irradiation in the absence of nucleotide excision repair and sho...
متن کاملNucleotide excision repair is a predominant mechanism for processing nitrofurazone-induced DNA damage in Escherichia coli.
Nitrofurazone is reduced by cellular nitroreductases to form N(2)-deoxyguanine (N(2)-dG) adducts that are associated with mutagenesis and lethality. Much attention recently has been given to the role that the highly conserved polymerase IV (Pol IV) family of polymerases plays in tolerating adducts induced by nitrofurazone and other N(2)-dG-generating agents, yet little is known about how nitrof...
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We investigated the relative contribution of the two Escherichia coli DNA alkyltransferases (ATases) to the increased sensitivity of ATase-deficient bacteria to the mutagenic and lethal effects of chloroethylnitrosoureas (CNU). The ogtencoded protein was the principal determinant in resistance to the mutagenic effects of CNU in E.coli. Thus, only when the ogt gene was inactivated was sensitivit...
متن کاملRole of the Escherichia coli nucleotide excision repair proteins in DNA replication.
DNA polymerase I (PolI) functions both in nucleotide excision repair (NER) and in the processing of Okazaki fragments that are generated on the lagging strand during DNA replication. Escherichia coli cells completely lacking the PolI enzyme are viable as long as they are grown on minimal medium. Here we show that viability is fully dependent on the presence of functional UvrA, UvrB, and UvrD (h...
متن کاملRole of DNA polymerases in excision repair in Escherichia coli.
The excision repair process following ultraviolet irradiation has been fractionated into its individual steps in toluene-treated cells. Incision can be examined in vitro independently of other reactions by omission of the deoxyribonucleoside triphosphates which prohibits repair synthesis and causes incisions to accumulate. Incision requires ATP, continues from 10 to 15 min at 37”, and is specif...
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ژورنال
عنوان ژورنال: Microbiological Reviews
سال: 1990
ISSN: 0146-0749
DOI: 10.1128/mr.54.1.18-51.1990